Proper spindle function is essential to maintain ploidy during cell division, and factors that influence spindle assembly and morphology are frequently altered in cancer ( Gordon et al., 2012). Spindle morphology varies across a wide range of cellular environments ( Helmke and Heald, 2014 Mitchison et al., 2015). The spindle is a complex and dynamic macromolecular machine that functions to accurately distribute chromosomes during eukaryotic cell division. The approaches outlined can be applied by other researchers studying spindle morphology and adapted with minimal modification to other experimental systems. Our analyses provide new insight into the diversity of spindle types and suggest areas for future study. We also apply our pipeline to analyze nuclear morphology in human cell culture, showing the general utility of the segmentation approach. Extract mixing experiments reveal that some spindle features titrate, while others undergo switch-like transitions, and multivariate analysis shows the pleiotropic morphological effects of modulating the levels of TPX2, a key spindle assembly factor. Our analysis of different spindle types illustrates how kinetochore microtubules amplify spindle microtubule density. Here, we present a large dataset of Xenopus extract spindle images together with an analysis pipeline designed to assess spindle morphology across a range of experimental conditions. However, few cell biologists possess the tools necessary to analyze them. You hereby agree to indemnify and keep indemnified F1000, its affiliates, contractors and agents from and against any and all losses (including without limitation direct, indirect and consequential loss), costs, claims, damages or expenses of whatever nature and howsoever caused arising directly or indirectly from any breach of these Terms and Conditions or arising from the Comments posted on this website or content contained in any email sent using the facilities provided by the website by you including without limitation as a result of any infringement of any intellectual property or other proprietary rights, libel, defamation, obscenity or the Comments being otherwise unlawful.Imaging datasets are rich in quantitative information.
#Cellprofiler measure object size shape license
You hereby grant to F1000 a non-exclusive royalty-free license to use, reproduce, edit and authorize others to use, reproduce and edit any of your Comments in any and all forms, formats or media. The Comments will not be used to solicit or promote business or custom or present commercial activities or unlawful activity.
#Cellprofiler measure object size shape software
The Comments do not contain any contaminating or destructive features or devices such as viruses, time bombs or coding designed to interrupt, destroy or limit the functionality of this website or any of this website's user's computer equipment or software and The Comments do not contain any defamatory, libelous, offensive, indecent or otherwise unlawful material or material which is an invasion of privacy The Comments do not infringe any intellectual property right, including without limitation copyright, patent or trademark, or other proprietary right of any third party
You are entitled to post the Comments on the website and have all necessary licenses and consents to do so To the extent permitted by applicable laws F1000 shall not be responsible or liable for the Comments or for any loss cost, liability, damages or expenses caused and or suffered as a result of any use of and/or posting of and/or appearance of the Comments on this website.į1000 reserves the right to monitor all Comments and to remove any Comments which it considers in its absolute discretion to be inappropriate, offensive or otherwise in breach of these Terms and Conditions.
Comments reflect the view and opinion of the person who posts such view or opinion. F1000 does not screen, edit, publish or review Comments prior to their appearance on the website and Comments do not reflect the views or opinions of F1000, its agents or affiliates. This Agreement shall begin on the date hereof.Ĭertain parts of this website offer the opportunity for users to post and exchange opinions, information, material and data ('Comments') in areas of the website. By clicking "I accept the User Comment Terms and Conditions" before you submit your first comment, you agree to be bound by these conditions every time you submit a comment. Submission of user comments to this website is subject to additional Terms and Conditions. Use of this website is subject to the F1000 Research Limited (F1000) General Terms and Conditions.